CAPÍTULOS DE LIVROS
MARTINS, MMI, SOUZA, FF, GOBELLO, C. Canine Transmissible Venereal Tumor: Etiology, Pathology, Diagnosis and Treatment. In: Patrick Concannon, Gary England, John Verstegen, Catharina Linde-Forsberg. (Org.). Recent Advances in Small Animal Reproduction, 2004.
http://www.ivis.org/advances/Concannon/gobello2/chapter.asp?LA=1
SOUZA, FF. Hiperplasia Prostática Benigna. In: Cristina Gobello. (Org.). Temas de Reproducción de Caninos y Felinos por Autores Latinoamericanos. 1ª ed. La Plata: Intermédica, 2004, p.71-83.
MARTINS, MIM, SOUZA, FF. Tumor Venéreo Transmisible. In: Cristina Gobello. (Org.). Temas de Reproducción de Caninos y Felinos por Autores Latinoamericanos. 1ª ed. La Plata: Intermédica, 2004, p.65-70.
TRABALHOS CIENTÍFICOS
FERNANDES, CES, SOUZA, FF, SOUZA-NETO, JA, RIBOLLA, PEM. Heparin-binding proteins of seminal plasma in Nellore bulls. Ciência Rural, v.39, p.275-278, 2009.
Resumo: O objetivo deste estudo foi identificar proteínas ligadoras à heparina no plasma seminal de touros Nelore (Bos taurus indicus). Para tanto, foram selecionados quatro touros entre 30 e 36 meses de idade e peso aproximado de 500-550kg. Após centrifugação, amostras do plasma seminal foram misturadas e as proteínas ligadoras à heparina foram isoladas por meio da cromatografia por afinidade. As frações após a eluição foram agrupadas para caracterização das bandas protéicas (SDSPAGE, 12,5%). Foram identificadas oito bandas protéicas variando entre 15 e 63kDa. Duas proteínas com 22 e 25kDa foram similares às descritas em touros Bos taurus taurus. Outras proteínas identificadas com 39, 53, 58 e 63kDa ainda não foram descritas e possivelmente sejam específicas para Bos taurus indicus.
http://www.scielo.br/pdf/cr/2008nahead/a44cr584.pdf
CARDOSO, RCS, SILVA, AR, SILVA, LDM, CHIRINÉA, VH, SOUZA, FF, LOPES, MD. Evaluation of fertilizing potential of frozen-thawed dog spermatozoa diluted in ACP -106 using an in-vitro sperm-oocyte interaction assay. Reproduction in Domestic Animals, v.42, p.11-16, 2007.
Abstract: The aim of present study was to evaluate frozen canine semen with ACP-106® (Powder Coconut Water) using an in vitro sperm–oocyte interaction assay (SOIA). Ten ejaculates from five stud dogs were diluted in ACP-106® containing 20% egg yolk, submitted to cooling in a thermal box for 40 min and in a refrigerator for 30 min. After this period, a second dilution was performed using ACP-106® containing 20% egg yolk and 12% glycerol. Samples were thawed at 38°C for 1 min. Post-thaw motility was evaluated by light microscopy and by using a computer aided semen analysis (CASA). Plasma membrane integrity and sperm morphology/acrosomal status were evaluated by fluorescent probes (C-FDA/PI) and Bengal Rose respectively. Moreover, frozen-thawed semen was analysed by a SOIA. Subjective post-thaw motility was 52.0 ± 14.8% and it was significant higher than the total motility estimated by CASA (23.0 ± 14.8%) because this system considered the egg yolk debris as immotile spermatozoa. Although normal sperm rate and acrosomal integrity evaluated by Bengal Rose stain was 89.6 ± 3.1% and 94.3 ± 3.1%, respectively, post-thaw percentage of intact plasma membrane was only 35.1 ± 14.3%. Regarding SOIA, the percentage of interacted oocytes (bound, penetrated and bound and/or penetrated) was 75.3%. Using regression analysis, it was found significant relations between some CASA patterns and data for SOIA. In conclusion, the freezing-thawing procedure using ACP-106® was efficient for maintain the in vitro fertility potential of dog spermatozoa.
http://www3.interscience.wiley.com/journal/118521997/abstract
SOUZA, FF, BARRETO, CS, LOPES, MD. Characteristics of seminal plasma proteins and their correlation with canine semen analysis. Theriogenology, v.68, p.1112-1123, 2007.
Abstract: The objectives were to separate canine seminal plasma proteins (with SDS-PAGE) and to determine the correlation between specific proteins and semen characteristics. Three ejaculates from 20 mixed-breed dogs, of unknown fertility, were collected by digital manipulation. Ejaculate volume and color, sperm motility, sperm vigor, percentage of morphologically normal spermatozoa, and membrane integrity (hypoosmotic swelling test and fluorescent staining) were assessed. For each dog, seminal plasma was pooled from all three ejaculates and proteins were separated with SDS-PAGE, using polyacrylamide concentrations of 13% and 22% in the separation gels. After staining, gel images were digitized to estimate molecular weights (MW) and integrated optical density (IOD) of each lane and of individual bands. Total seminal plasma protein concentration was 2.19 ± 1.56 g/dL (mean ± SD, range 1.12–5.19 g/dL). A total of 37 protein bands were identified (although no dog had all 37 bands). In the 13% gel, molecular weights ranged from 100.6 to 17.1 kDa, with four bands (49.7, 33.2, 26.4, and 19.5 kDa) present in samples from all dogs. In the 22% gel, molecular weights ranged from 15.6 to 3.6 kDa, with nine bands (15.6, 13.5, 12.7, 11.7, 10.5, 8.7, 7.8, 5.6, and 4.9 kDa) present in samples from all dogs. Combined for both gels, the majority of bands (85%) had molecular weights <17 kDa, with B20 (15.6 kDa) in high concentrations in samples from all dogs. There were positive correlations (P ≤ 0.01) between two bands, B4 (67 kDa) and B5 (58.6 kDa), and sperm motility (r = 0.66 and r = 0.46), sperm vigor (r = 0.56 and r = 0.66), percentage of morphologically normal spermatozoa (r = 0.55 and r = 0.59), the hypoosmotic swelling test (r = 0.76 and r = 0.68), and fluorescent staining (r = 0.56 and r = 0.59), respectively. In conclusion, 37 proteins were identified in seminal plasma, two were significantly correlated with semen characteristics.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCM-4NSPVS3-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=bda6939b0511f8e544b3d27d2edd6659
SOUZA, FF. Proteínas do sêmen do cão são importantes ou não na fertilização? Revista Brasileira de Reprodução Animal, v. 31, p. 108-114, 2007.
Resumo: Proteínas presentes no plasma seminal se ligam à membrana espermática durante a ejaculação e podem ser determinantes da fertilidade de um macho. No cão, poucos estudos têm demonstrado seus efeitos nas características seminais e na fertilidade, porém em várias outras espécies tem sido demonstrada a importância de diferentes proteínas na fertilização. Estudos das proteínas do plasma seminal ou na membrana espermática podem ser úteis na identificação de animais férteis, na elaboração de aditivos seminais e de contraceptivos. Ademais, informações obtidas a respeito da fisiologia da célula espermática e do envolvimento das proteínas na fertilidade de cães podem ser aplicadas à biotecnologia da reprodução de animais silvestres.
http://www.unb.br/posgraduacao/docs/fav/Proteinassemencaofertilizacao.pdf
TEBET, JM, MARTINS, MIM, CHIRINÉA, VH, SOUZA, FF, CAMPAGNOL, D, LOPES, MD. Cryopreservation effects on domestic cat epididymal versus electroejaculated spermatozoa. Theriogenology, v.66, p.1629-1632, 2006.
Abstract: The aims were to evaluate the susceptibility of feline ejaculated and epididymal spermatozoa to cold shock and to evaluate the effect of egg yolk in the preservation extender. Ejaculated and epididymal spermatozoa from eight males were subjected to a slow (0.5 °C/min) or a fast (3 °C/min) cooling rate with controls kept in room temperature. Ejaculated and epididymal spermatozoa from another eight males were cooled in a plain Tris buffer (Tris) or in Tris with 20% egg yolk (EYT) and evaluated for 96 h. Subjective motility (MOT), plasma membrane integrity (PMI), and acrosome integrity (ACRI) were evaluated. Cooling did not induce sperm damage regarding PMI (P = 0.6) or ACRI (P = 0.19) and chilled spermatozoa had better overall MOT (P = 0.046) than controls. EYT was better for MOT (P > 0.05) from 48 h of cold storage than Tris. EYT was also better for overall ACRI (P < 0.0001) while Tris was better for overall PMI (P = 0.0004). There were no interactions between time and treatment (P > 0.05) for PMI or ACRI. Ejaculated spermatozoa had better overall MOT (P < 0.05) and PMI (P < 0.05) than epididymal spermatozoa, and higher ACRI in experiment 1 (P = 0.0003) but not in experiment 2 (P = 0.117). Source of spermatozoa did not affect the susceptibility to cooling or the effect of egg yolk as there were no interactions (P > 0.05) between source of spermatozoa and treatment (cooling or control) or between time, source and extender (P > 0.05). In conclusion cat spermatozoa were tolerant to cold shock and egg yolk was beneficial for preservation of MOT and ACRI but not PMI.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCM-4N6Y5F8-1&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=bef181fcf1ae2394b28f910ff9044643
SILVA, AR, CARDOSO, RCS , SILVA, LDM, CHIRINÉA, VH, LOPES, MD, SOUZA, FF. Prognostic value of canine frozen-thawed semen parameters on in vitro sperm-oocyte interactions. Theriogenology, v.66, p.456-462, 2006.
Abstract: Combining the data from conventional semen analysis with oocyte penetration assays should improve the assessment of the fertilizing ability of a semen sample. Thus, the objective of the present study was to evaluate the prognostic value of various semen parameters on the in vitro interactions between frozen-thawed canine sperm and homologous oocytes. Ten ejaculates from five stud dogs (two ejaculates/dog) were collected by digital manipulation. Semen samples were evaluated, extended in Tris–egg yolk–glycerol, frozen and stored in liquid nitrogen, and thawed several weeks later. Samples were evaluated for motility and sperm populations by computer-aided semen analysis (CASA), plasma membrane integrity (carboxy-fluorescein diacetate and propidium iodide), and sperm morphology (Bengal Rose). Thawed spermatozoa were also incubated with homologous oocytes for 18 h in an atmosphere of 5% CO2 and 95% air at 38 °C and sperm–oocyte interactions were evaluated. Simple linear regression models were calculated, with sperm parameters as independent variables and sperm–oocyte interactions as the dependent variable. There were significant associations between: percentage of oocytes bound to spermatozoa and beat cross frequency (BCF, R2 = 63%), percentage of oocytes that interacted with spermatozoa and BCF (R2 = 73%), and number of penetrated spermatozoa and velocity average pathway (VAP, R2 = 64%) and velocity straight line (VSL, R2 = 64%). Although plasma membrane integrity and sperm morphology had little prognostic value for in vitro interactions between canine frozen-thawed sperm and homologous oocytes, some motility patterns (evaluated by CASA) were predictive of in vitro sperm–oocyte interactions.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCM-4J2W08V-1&_user=10&_coverDate=07%2F15%2F2006&_alid=881029065&_rdoc=2&_fmt=high&_orig=search&_cdi=5174&_sort=d&_docanchor=&view=c&_ct=8&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=fb02a76edfb8bda28fd96d857f861b29
SOUZA, FF, MARTINS, MIM, FERNANDES, CES, RIBOLLA, PEM, LOPES, MD. Heparin-binding proteins of canine seminal plasma. Theriogenology, v.66, p.1606-1609, 2006.
Abstract: Heparin-binding proteins (HBP) from seminal plasma have been expected to participate in modulation of the acrosomal reaction, and have been correlated with fertility in some species. However, they have not been described in the dog. The aim of this study was to document the HBPs of canine seminal plasma. Six pooled samples of seminal plasma from three crossbred dogs were used. The HBPs were isolated by heparin affinity chromatography and the fractions recovered were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was carried out on 12 and 18% vertical minigels. The stained gels were scanned and the molecular weight (kDa) values for each band within a lane were calculated by image analysis software. The electrophoresis analysis of the pooled eluded fractions identified 19 bands, with molecular weights varying from 61.5 to 5.2 kDa. Previous studies, using one-dimensional SDS-PAGE, identified two bands (67 and 58.6 kDa), which were positively correlated with some semen parameters (sperm motility, sperm vigor, percentage of morphologically normal sperm and plasma membrane integrity). The 61.5 kDa band detected in the present study apparently corresponded to the 58.6 kDa band identified previously. Canine seminal plasma contained HBP, since HBP modulate the acrosome reaction in other species, they may have the same function in the dog. Further studies are necessary to better characterize this protein and determine if it is associated with fertility in the dog.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCM-4K5JVNF-1&_user=10&_coverDate=10%2F31%2F2006&_alid=881031467&_rdoc=35&_fmt=high&_orig=search&_cdi=5174&_sort=d&_docanchor=&view=c&_ct=235&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=b1c359531b30e70b49dfcd4d323491fb
SOUZA, FF, MARTINS, MIM, LOPES, MD. Vasectomy effect on canine seminal plasma biochemical components and their correlation with seminal parameters. Theriogenology, v.66, p.1621-1625, 2006.
Three semen samples were collected at 48 h intervals from 20 mature research dogs previously conditioned to manual semen collection. Vasectomy was performed in all dogs, and 15 days after surgery, another three ejaculates were similarly collected. The semen was evaluated, and centrifuged to obtain seminal plasma for measurement of pH, and concentrations of total proteins (TP), total chlorides (Cl), calcium (Ca), potassium (K), and sodium (Na). The seminal plasma protein profile was evaluated by SDS-PAGE, molecular weights and the integrated optical density (IOD) of each band were estimated. There was a negative correlation between K concentration and progressive motility (r = −0.49, P = 0.027), sperm vigor (r = −0.60, P = 0.0053), and plasma integrity, evaluated by both the hypo-osmotic swelling test (r = −0.50, P = 0.026) and a fluorescent stain (r = −0.45, P = 0.046). Positive correlations between Na and K pre- and post-vasectomy (r = 0.88, P < 0.001, r = 0.56, P < 0.01, respectively) were verified. There were a total of 37 bands pre-vasectomy and 35 post-vasectomy (range, 100.6–3.6 kDa). Bands B9 and B13 (42.6 and 29.2 kDa) were not present post-vasectomy. The IOD of band B3 (73.5 kDa) was higher (P = 0.03) pre-vasectomy, compared to post-vasectomy, conversely, the IODs of bands B29 and B37 (7.8 and 3.6 kDa) increased (P = 0.026 and 0.047). Pre-vasectomy, there was a positive correlation (r = 0.49, P = 0.029) between band B37 band (3.6 kDa) and the Na:K ratio. In conclusion, K appeared to be involved in sperm motility in dogs and could be a tool to evaluate sperm function. The prostate contributed several elements to canine seminal plasma. Vasectomy changed Ca concentrations and the protein profile of the seminal plasma. Further studies must be performed to clarify the function of these elements on the in vivo fertility of dogs.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCM-4JTR8VY-1&_user=10&_coverDate=10%2F31%2F2006&_alid=881031467&_rdoc=36&_fmt=high&_orig=search&_cdi=5174&_sort=d&_docanchor=&view=c&_ct=235&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c28b1ba4be7ce804bac73ea7947e6977
MARTINS, MIM, SOUZA, FF, OBA, E, LOPES, MD. The effect of season on serum testosterone concentration in dogs. Theriogenology, v.66, p.1603-1605, 2006.
Abstract: Photoperiod and environmental temperature are two important factors that may influence the reproductive cycle of various species. The objective of this study was to investigate seasonal influences on serum testosterone concentrations in dogs in a tropical zone, where the variation in day length between winter and summer solstice was approximately 2.5 h. Blood samples were collected every 15 days from seven adult dogs over a 14-month interval and serum testosterone concentrations were determined by radioimmunoassay. The year was divided into four seasons and mean testosterone concentrations for each season were related to the mean environmental temperature and rainfall during that season. Mean testosterone concentrations were 1.81 ng/mL (winter 2002), 1.93 ng/mL (spring 2002), 1.31 ng/mL (summer 2003), 2.02 ng/mL (autumn 2003) and 1.93 ng/mL (winter 2003). The temperature ranged from 10.2 to 32.8 °C and the rainfall from 33 to 476 mm. Serum testosterone concentrations were lower in summer 2003 than in both spring 2002 (P = 0.05) and autumn 2003 (P = 0.016). In a tropical zone, a combination of high temperature and substantial rainfall may have reduced serum testosterone concentrations in dogs.
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCM-4K7FJ7S-1&_user=10&_coverDate=10%2F31%2F2006&_alid=881039135&_rdoc=87&_fmt=high&_orig=search&_cdi=5174&_sort=d&_docanchor=&view=c&_ct=583&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=69077ffbe4251b5478afedac9c8583fc
CHIRINÉA, VH, MARTINS, MIM, SOUZA, FF, TEBET, JM, PAPA, FO, LOPES, MD. Características morfofuncionais do sêmen canino refrigerado e congelado, usando dois diferentes meios diluentes. Ciência Animal Brasileira (UFG), v.7, p.407-415, 2006.
Resumo: O objetivo deste estudo foi avaliar, por meio de análise funcional e morfológica, dois meios diluentes – TRIS/frutose/ácido cítrico/glicerol (8 %) (TRIS 8 %) (Morton, 1988 modificado) e um meio comercial (MP50) (PAPA et al., 2002) – para criopreservação de sêmen canino. Colheram-se dez ejaculados de cães adultos, por manipulação digital do pênis. Avaliaram-se as amostras pela motilidade espermática, velocidade espermática, teste hiposmótico, integridade de membrana espermática, morfologia espermática, e análise ultra-estrutural no sêmen fresco (T1), refrigerado (T2) e descongelado (T3). Envasaram-se as amostras diluídas em palhetas francesas de 0,5 mL, com 40 x 106 espermatozóides/palheta e se as mantiveram por sessenta minutos a 5 ºC (T2), em seguida, transferiram-nas para vapor de nitrogênio líquido durante vinte minutos e posteriormente estocadas. O sêmen foi descongelado a 70°C por 8 segundos. A análise de variância mostrou influência do animal nas diferentes variáveis, com exceção da motilidade espermática e integridade de membrana espermática. Na refrigeração (T2), o meio MP50 apresentou melhores resultados no teste hiposmótico e na integridade de membrana (p<0,05) e no sêmen descongelado (T3) não foi observada diferença significativa entre os meios (p>0,05). A análise ultra-estrutural do sêmen mostrou edema e ondulação da membrana plasmática e acrossomal nas diferentes etapas do processo de criopreservação. Conclui-se que os meios diluentes utilizados mostraram ser semelhantes quanto às características morfofuncionais após a descongelação.
http://revistas.ufg.br/index.php/vet/article/viewFile/870/1009
SOUZA, FF, MARTINS, MIM, CARVALHO, FCA, AIRES, MB, OBA, E. Bubaline aminiotic and allantoic fluid protein profile. Buffalo Journal, v.3, p.225-232, 2004.
Abstract: The study of amniotic and allantoic fluid composition can be important to reflect the foetal metabolism and development. Like this bubaline may be an important experimental model to study the physiology of molecule transport between placental compartments. The objective of this study was to evaluate total protein concentration and to describe electrophoretic profile of amniotic and allantoic fluids correlating them with length and sex of bubaline foetuses. The fluids were collected from 17 bubaline genitals by puncture of amniotic and allantoic sacks. Total protein concentration was determined using bicinchoninic acid method and the protein profile by SDS polyacrilamide gel electrophoresis. The gels were stained with silver nitrate and digital images were analysed. Total protein concentration was significantly lower (p=0.0002) in the allantoic fluid. There was a positive correlation (r=0.77, p=0.0003) between its total protein concentration and foetal crown-rump length. A total of 39 and 44 bands were found in the allantoic and amniotic fluids, respectively. There was no correlation between foetus sex and the other variables. We concluded that protein composition in the amniotic and allantoic fluid is different, and allantoic fluid proteins correlate with foetal development.
http://www.cababstractsplus.org/abstracts/Abstract.aspx?AcNo=20053100687
SOUZA, FF, LEME, DP, UECHI, E, LOPES, MD. Evaluation testicular fine needle aspiration cytology and serum testosterone levels in dogs. Brazilian Journal of Veterinary Research and Animal Science, v.41, p.98-105, 2004.
Abstract: In order to verify alternative clinical approach in the reproductive evaluation of 4 adult dogs, the fine needle aspiration cytology (FNAC) and serum testosterone levels were used together with testicular volume measurement and semen analysis. FNAC was performed in both testes and serum testosterone concentrations were assayed in regular intervals during a 24h period. Results of semen analysis and FNAC were normal in dogs 1 and 3. In dog 2, small testes, poor semen quality, a high percentage of Sertoli cells and early spermatids were found suggesting testicular degeneration. In dog 4, a small right testicle, poor semen quality with low sperm concentration and an uncounted amount of spermatozoa in the FNAC indicated testicular degeneration due to an obstructive lesion, whereas high percentage of distal droplets, thicker left epididymis and normal FNAC of the left testis suggested a slow sperm transit. Testosterone circadian rhythm was clear in 3 of 4 dogs, although concentrations were low. Testicular volume, semen analysis and testicular FNAC could provide valuable information about spermatogenesis. In contrast, serum testosterone concentration was not clearly correlated with any reproductive characteristic of those dogs.
http://www.scielo.br/pdf/bjvras/v41n2/25225.pdf
ROMAGNOLI, S, SOUZA, FF, ROTA, A, VANNOZZI, I. Prolonged interval between parturition of normal live pups in a bitch. Journal of Small Animal Practice, v.45, n.5, p.249-253, 2004.
Abstract: On day 64 after artificial insemination, a six-year-old primiparous briard bitch whelped three live pups between 05.00 and 08.00. It was presented at 11.00 on the same day with failure to complete parturition. On ultrasound examination, a normal live fetus was observed and the bitch was treated with oxytocin three times during the day (1.0, 2.0 and 2.0 iu intramuscularly), with no effect. The following day, a higher dose of oxytocin (5.0 iu) was administered intramuscularly at 11.00, after a uterine ultrasound examination confirmed viability of the fetus. At 18.00 of the same day, the bitch whelped the fourth normal live pup, 37 hours after initiation of parturition and 34 hours after expulsion of the last fetus. Effectiveness of oxytocin and normal versus prolonged parturition due to uterine inertia are discussed.
http://www3.interscience.wiley.com/journal/118759097/abstract
SOUZA, FF, TONIOLLO, GH, TRINCA, LA. Avaliação do tamanho prostático de cães normais por meio da ultra-sonografia. Ars. Veterinaria, Jaboticabal, v.18, n.3, p.204-209, 2002.
Resumo: O objetivo deste estudo foi avaliar a próstata de 32 cães, sem sinais aparentes de enfermidades prostáticas, por palpação retal e exame ultra-sonográfico de acordo com a faixa etária (grupo I, cães até 3 anos, grupo II, cães de 3 a 6 anos, e grupo III, cães acima de 7 anos de idade) e peso corporal. Os resultados obtidos não mostraram correlação significativa entre os grupos etários, a localização, a forma, o comprimento e a altura prostática. Contudo, existiu uma correlação significativa (p<0.001) entre o peso corporal, o comprimento e a altura, sendo possível determinar um intervalo de confiança para os valores esperados dessas duas medidas e confeccionar uma equação (Comprimento (cm) = 2.60 + 0.10 x Peso (Kg), e Altura (cm) = 1.85 + 0.09xPeso (Kg)) onde pode ser encontrada uma estimativa para o valor médio de acordo com o peso corporal do cão.
CRESPILHO, AM, MARTINS, MIM, SOUZA, FF, LOPES, MD, PAPA, FO. Abordagem terapêutica do paciente neonato canino e felino: 1. Particularidades farmacocinéticas. Revista Brasileira de Reprodução Animal, v.30, n.1/2, p.3-10, 2006.
Resumo: Durante os primeiros meses de vida, filhotes caninos e felinos apresentam um grau variável de imaturidade dos sistemas fisiológicos que se desenvolvem durante as diferentes fases de crescimento neonatal, culminando com a maturidade orgânica inerente aos indivíduos adultos. Ao longo do desenvolvimento, diferenças marcantes são observadas na resposta dos filhotes ao uso de medicamentos, sobretudo no tocante à farmacocinética neonatal, representada por padrões diferenciais de absorção, distribuição, metabolização e excreção de fármacos em relação aos cães e gatos adultos. Frente ao exposto, o objetivo da presente revisão é abordar as principais particularidades fisiológicas dos filhotes, que exercem influência sobre a farmacocinética neonatal, apresentando os conceitos que norteiam a correta abordagem terapêutica do filhote canino e também do felino.
http://www.cbra.org.br
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